Modeling human brain development and developmental diseases using hiPSCs
题目：Modeling human brain development and developmental diseases using hiPSCs
讲座人：Guo-li Ming, PhD
Perelman Professor of Neuroscience
University of Pennsylvania
Human Induced pluripotent stem cells (hiPSCs) has the potential to generate all cell types of a human body under 2D culture conditions or form organ like structures- organoids, under 3D culture conditions. Cerebral organoid cultures from human iPSCs have been recently developed to recapitulate the cytoarchitecture of the developing brain. These hiPSC based model systems offer unique advantages in understanding molecular and cellular mechanisms governing embryonic neural development and in modeling neurodevelopmental disorders, such as brain malformation and neuropsychiatric disorders. We have improved the organoid technology and developed a protocol to produce forebrain-specific organoids derived from hiPSCs using a novel miniaturized spinning bioreactor that recapitulate the human embryonic cortical development. I will discuss our recent work using these systems to understand ZIKV induced microcephaly and mental disorders.